Home > Group > Instrument > GC Guideline

Guidelines for using Gas Chromatograph

Before:

  1. Make sure that you have the necessary training and permission from the person incharge (PH: pl2023@columbia.edu)

  2. You should only be using the column assigned to you. Not other columns for any reason.

  3. Please let the column in charge know that you plan to use the system.

  4. Login to the logbook. (Failure to do so would result in loosing the GC and GC/MS usage privileges)

During:

  1. First login using the appropriate screensaver password if the screensaver is on.

  2. Open the air and hydrogen cylinders (if not open already for use by someone else).

  3. Increase the head pressure to the appropriate value.

  4. Make sure that nobody is using the other GC in the same line. (if the other GC is running then wait till the run gets over)

  5. Open the air and hydrogen valves for the appropriate detector. Then press that ignition button to turn on the detector. You should see a signal of around 40.

  6. Load your method by going to the menu bar Method ‡ Load .

  7. Then go to the menu bar Runcontrol ‡ Sample info and input the appropriate information about the sample.

  8. It is important to update the sample info, other wise you could overwrite someone else’s file and please remember how you would feel if the same thing happens to you.

  9. Select your appropriate directory to store your data.

After:

  1. After the run is over, set the oven temperature to 40 and lower the head pressure to 5.

  2. Close the air and hydrogen cylinders (if no one else is using the other GC).

  3. Never touch the helium cylinder controls.

  4. Logout in the logbook.

Sample guidelines

  1. Never leave the samples next to the instrument.

  2. There would be a cleanup of the surroundings every morning and all the samples found around the instrument would be trashed.

  3. If I continue to find samples from a person, the GC and GC-MS privileges would be suspended for a certain period of time.

  4. Keep the sample concentration at nanogram level.

Storage and Maintanence of data

  1. Please pickup your trace immediately after the run is over.

  2. Printed data would be kept in a assigned spot for one more day for you to pickup. After that it would be trashed. Please do not blame me if the data is lost.

  3. Also store the data in the computer in your subfolder in the data directory.

  4. Please backup your data for long-term storage.

FOR USING GC-MS, Please get trained from PH

NOBODY IS ALLOWED TO USE THE GC OR GC/MS WITHOUT THE PERMISSION OF THE PERSON INCHARGE. ONLY THE PERSON INCHARGE CAN TRAIN AND PERMIT THE USAGE OF GC AND GC/MS. NON-TURRO GROUP PEOPLE SHOULD GET PERMISSION FROM PROF. TURRO BEFORE THEY COULD BE ALLOWED USAGE OF THE INSTRUMENTS. NO EXCEPTIONS.